Product: Anti-ARC (C-terminus), Polyclonal
Cat. No: PC-036 (100 μg)
Background:
Apoptosis is regulated by death domain (DD)
and/or caspase recruitment domain (CARD)
containing molecules and a caspase family of
proteases. CARD domain containing cell death
regulators include RAIDD, Apaf-1, caspase-9,
and caspase-2. A novel CARD domain
containing protein was recently identified and
designated ARC for apoptosis repressor with
CARD (1). ARC interacts with caspase-2 and -8
and inhibits enzymatic activity of caspase-8.
ARC suppresses apoptosis induced by cell
death adapters FADD and TRADD and by cell
death receptors Fas, TNFR-1 and DR3. The
messenger RNA of ARC is primarily expressed
in skeletal muscle and cardiac tissue (1).
Specificity:
This antibody binds ARC.
Positive control: Whole cell lysate from HeLa
cells.
Species Reactivity:
Human, others not tested.
Ig Isotype: IgG
Immunogen:
A peptide corresponding to amino acids 191 to
208 of human ARC (1).
Format:
Immunoaffinity chromatography purified IgG,
100 μg in 200 μl of PBS containing 0.02%
sodium azide.
Storage and Stability:
Stable for at least 12 months when stored at 4°C.
Applications and Suggested Dilutions:

Western blotting: Suggested dilution: 1:1000.
The optimal dilution for a specific application
should be determined by the researcher.
Reference:
1. Koseki T, Inohara N, Chen S, Nunez G. (1998)
ARC, an inhibitor of apoptosis expressed in
skeletal muscle and heart that interacts
selectively with caspases. Proc. Natl. Acad.
Sci. U S A 195:5156-5160.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.