ETFA抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
ETFA抗体

ETFA抗体

商家询价

产品名称： ETFA抗体

英文名称： ETFAAntibody

产品编号： XY12262-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
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 ETFA抗体

Applications
In-house applications ELISA, WB, IHC, IF, FC     Application not tested? Please see our Guarantee.
Published applications WB Publications 3 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 12262-1-AP;mouse small intestine tissuemouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP(ETFA antibody) at dilution of 1:1000
  • 12262-1-AP;Breast cancerImmunohistochemical of paraffin-embedded human breast cancer using 12262-1-AP(ETFA antibody) at dilution of 1:50 (under 40x lens)
  • 12262-1-AP;MCF-7Immunofluorescent analysis of MCF-7 cells, using PNRC2 antibody 12262-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 12262-1-AP;MCF-7 cells1X10^6 MCF-7 cells were stained with 0.2ug ETFA antibody (12262-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Positive WB detected in Mouse small intestine tissue, human ileum tissue, Y79 cells
Positive IHC detected in Human breast cancer tissue
Positive IF detected in MCF-7 cells
Positive FC detected in MCF-7 cells
Recommended dilution WB : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
 ETFA抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen ETFA fusion protein ag2931 Full name Electron-transfer-flavoprotein, alpha polypeptide
Calculated molecular
weight		 30 kDa Observed molecular 
weight		 30 kDa
GenBank accession 
number		 BC015526 Gene ID (NCBI) 2108
Gene symbol ETFA Synonyms Alpha ETF, EMA, ETFA, GA2, MADD
Background ETFA抗体

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. ETFA抗体

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