产品名称：小鼠胚胎成纤维细胞

货号：M7570
 

Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells [1]. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages [2]. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.

MEF from ScienCell Research Laboratories are isolated from embryonic day 13 CF1 mouse embryos. These cells are cryopreserved at P0 and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. MEF are characterized by immunofluorescence with antibody specific to fibronectin. MEF are negative for mycoplasma, bacteria, yeast, and fungi. MEF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use DMEM (Cat. No. 09221) containing 10% fetal bovine serum (FBS, Cat.No.0010, 0025, 0500) for the culturing of MEF cells in vitro.