RAB27A抗体Applications
| In-house applications |
ELISA, WB, IHC, IF, IP Application not tested? Please see our Guarantee. |
| Published applications |
WB |
Publications |
6 citations –view here |
| Species specificity |
Human; other species not tested. |
| Published Species |
Chicken, human, mouse, rat |
|
Images
Prev12Next
 HL-60 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP(RAB27A antibody) at dilution of 1:1000 Immunohistochemical of paraffin-embedded human liver cancer using 17817-1-AP(RAB27A antibody) at dilution of 1:100 (under 10x lens) Immunohistochemical of paraffin-embedded human liver cancer using 17817-1-AP(RAB27A antibody) at dilution of 1:100 (under 40x lens) Immunofluorescent analysis of HepG2 cells, using RAB27A antibody 17817-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). IP Result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 3200ug.
|
| Positive WB detected in |
HL-60 cells, Apoptosised HeLa cells, HeLa cells, human liver tissue, Jurkat cells, K-562 cells, L02 cells |
| Positive IP detected in |
K-562 cells |
| Positive IHC detected in |
Human liver cancer tissue, human kidney tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100 |
RAB27A抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information RAB27A抗体
| Immunogen |
RAB27A fusion protein ag12136 |
Full name |
RAB27A, member RAS oncogene family |
Calculated molecular
weight |
221aa,25 kDa |
Observed molecular
weight |
25-27 kDa |
GenBank accession
number |
BC107680 |
Gene ID (NCBI) |
5873 |
| Gene symbol |
RAB27A |
Synonyms |
GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A |
Background
|
Rab27a is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27a is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27a is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal. RAB27A抗体
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