驱动蛋白家族成员18A(KIF18A)重组蛋白

YB518Hu012
Recombinant Kinesin Family, Member 18A (KIF18A)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
驱动蛋白家族成员18A(KIF18A)重组蛋白[ PROPERTIES ]
Residues: Met1~Lys453
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q8NI77
Host: E. coli
Subcellular Location: Cell projection, ruffle.
Cytoplasm. Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
驱动蛋白家族成员18A(KIF18A)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.6
Predicted Molecular Mass: 55.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
驱动蛋白家族成员18A(KIF18A)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE VSFFHGKKTT
NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS FLNGYNCTVL AYGATGAGKT
HTMLGSADEP GVMYLTMLHL YKCMDEIKEE KICSTAVSYL EVYNEQIRDL LVNSGPLAVR
EDTQKGVVVH GLTLHQPKSS EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD
KTASINQNVR IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR
KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN RAKDIKSSLK
SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF TNENDQAKLM ISNPQEKEIE
RFQEILNCLF QNREEIRQEY LKLEMLLKEN ELK