ENC1,外胚层神经皮层蛋白1/KLHL35抗体

产品编号HZ-8043R
英文名称ENC1
中文名称外胚层神经皮层蛋白1/KLHL35抗体
别 名CCL 28; CCL28; Ectoderm neural cortex protein 1; Ectoderm-neural cortex protein 1; Ectodermal neural cortex (with BTB like domain); ENC 1; ENC-1; ENC1; ENC1_HUMAN; FLJ39259; Kelch like 35; Kelch like 37; Kelch-like protein 37; KLHL 35; KLHL35; KLHL37; NRPB; Nuclear matrix protein NRP/B; Nuclear restricted protein BTB domain like (brain); p53 induced gene 10 protein; p53-induced gene 10 protein; PIG 10; PIG10; TP53I10; Tumor protein p53 inducible protein 10.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学 细胞类型标志物 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,
ENC1,外胚层神经皮层蛋白1/KLHL35抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量66kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ENC1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ENC1,外胚层神经皮层蛋白1/KLHL35抗体PubMedPubMed
产品介绍background:
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificity: Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas.

Function:
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Subunit:
Binds to RB1. Hypophosphorylated RB1 associates with ENC1 during neuronal differentiation, while hyperphosphorylated RB1 associates with ENC1 in undifferentiating cells. Part of a complex that contains CUL3, RBX1 and ENC1.

Subcellular Location:
Nucleus matrix. Cytoplasm, cytoskeleton.

Tissue Specificity:
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas.

Post-translational modifications:
Ubiquitinated and probably targeted for proteasome-independent degradation.

Similarity:
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.

Database links:
UniProtKB/Swiss-Prot: O14682.2

ENC1,外胚层神经皮层蛋白1/KLHL35抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.