UBE2E3 polyclonal antibody
产品名称： UBE2E3 polyclonal antibody
英文名称： UBE2E3 polyclonal antibody
产品编号： PAB1771
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of UBE2E3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human UBE2E3.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin-conjugating enzyme.
Ito K, Kato S, Matsuda Y, Kimura M, Okano Y.Cytogenet Cell Genet. 1999;84(1-2):99-104.
- Applications
- Western Blot (Tissue lysate)
- UBE2E3 polyclonal antibody (Cat # PAB1771) is used in Western blot to detect UBE2E3 in mouse liver tissue lysate.
- Western Blot (Cell lysate)
- (RIGHT) Western blot analysis of UBE2E3 polyclonal antibody (Cat # PAB1771) in Y-79 cell line lysates (35 ug/lane). UBE2E3 (arrow) was detected using the purified polyclonal antibody.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with UBE2E3 polyclonal antibody (Cat # PAB1771) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 10477
- Protein Accession#:
- Q969T4
- Gene Name:
- UBE2E3
- Gene Alias:
- UBCH9,UbcM2
- Gene Description:
- ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
- Omim ID:
- 604151
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
- Other Designations:
- ubiquitin carrier protein,ubiquitin-conjugating enzyme E2E 3,ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5),ubiquitin-protein ligase
- Gene Pathway
- Related Disease