DISC1-LONG-SPECIFIC 抗体
Applications
| Tested Applications: |
ELISA, WB, IHC, IF, IP |
| Specificity Reaction: |
Human, Mouse; Other species are not tested.Please decide the specificity by homology |
| Positive WB detected in |
SH-SY5Y cells, human kidney tissue, mouse brain tissue, mouse kidney tissue |
| Positive IP detected in |
Mouse brain tissue |
| Positive IHC detected in |
Human brain tissue |
| Positive IF detected in |
MDCK cells |
| Recommended dilution |
WB : 1:200-1:1000
IP : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:20-1:200 |
DISC1-LONG-SPECIFIC 抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15500-1-AP(DISC1-long-specific antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human brain using 15500-1-AP(DISC1-long-specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using 15500-1-AP(DISC1-long-specific antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-DISC1-long-specific (IP:15500-1-AP, 4ug; Detection:15500-1-AP 1:300) with mouse brain tissue lysate 2560ug.
Immunofluorescent analysis of MDCK cells using 15500-1-AP( DISC1-long-specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Product Information DISC1-LONG-SPECIFIC 抗体
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information
| Immunogen: |
Peptide |
Full Name: |
Disrupted in schizophrenia 1 |
Caculated Molecular
Weight: |
95 kDa |
Observed Molecular
Weight: |
60kDa, 62 kDa,75- 85 kDa |
| GenbankNo |
NM_018662 |
GeneID: |
27185 |
| Symbol |
DISC1 |
Synonyms |
C1orf136, DISC1, DISC1 long, disrupted in schizophrenia 1, FLJ13381, FLJ21640, FLJ25311, FLJ41105, KIAA0457, SCZD9 |
Background
|
DISC1, also named as KIAA0457, is identified as the sole gene whose ORF is truncated and cosegregates with major mental illnesses in a Scottish family. DISC1 may be implicated in psychiatric conditions in other populations than the unique Scottish family. It is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. It plays a role, together with PCNT, in the microtubule network formation. In WB, DISC1 has some MW such as 34kd(PMID:18620078 );75-85kd and 95-100kd(PMID:15657124). DISC1 isoforms have distinct developmental patterns of expression. This antibody is against C-terminal of DISC1, binding full length protein specifically. DISC1-LONG-SPECIFIC 抗体
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