前脂肪细胞生长因子
产品名称： 前脂肪细胞生长因子
英文名称： Preadipocyte Growth Supplement
产品编号： 7252
产品价格： 0
产品产地： 美国
品牌商标： scienell
更新时间： 2024-10-28T10:24:25
使用范围： null
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- 地址 : 上海市宝山区长江南路180号A区401-406室
- 邮编 : 200433
- 所在区域 : 上海
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Product Description Preadipocyte Growth Supplement (PAGS) is a medium supplement designed for the optimal growth of normal human preadiopocytes in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal human preadipocytes. The supplement is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced growth environment that maximally promotes the growth of normal human preadipocytes in vitro. The supplement is designed as an additive for preadipocyte medium (PAM, Cat. No. 7211) and should be used in conjunction with that medium. Components PAGS is packaged in the quantity of supplement suited for a 500 ml bottle of Preadipocyte Medium. When a 500 ml bottle of preadipocyte medium is supplemented with PAGS, the final concentrations of the supplement components per milliliter will be BSA 10 μg, apo-transferrin 10 μg, insulin 5 mg, EGF 2 ng, FGF-2 2 ng and hydrocortisone 1 mg. Product Use PAGS is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Store the PAGS at -20oC before adding to Preadipocyte Medium. Shipping Dry ice. Prepare for use Thaw PAGS at 37oC. Gently tilt the PAGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add PAGS and other components (FBS and P/S solution) into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of Preadipocyte Medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
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