UBB polyclonal antibody
产品名称： UBB polyclonal antibody
英文名称： UBB polyclonal antibody
产品编号： PAB2409
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of UBB.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human UBB.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The carboxyl extensions of two rat ubiquitin fusion proteins are ribosomal proteins S27a and L40.
Chan YL, Suzuki K, Wool IG.Biochem Biophys Res Commun. 1995 Oct 13;215(2):682-90.
- 2.
- Differential feeding-related regulation of ubiquitin and calbindin9kDa in rat duodenum.
Hubbard MJ, Carne A.Biochim Biophys Acta. 1994 Jul 6;1200(2):191-6.
- 3.
- Structure of tetraubiquitin shows how multiubiquitin chains can be formed.
Cook WJ, Jeffrey LC, Kasperek E, Pickart CM.J Mol Biol. 1994 Feb 18;236(2):601-9.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of UBB polyclonal antibody (Cat # PAB2409) in HeLa cell lysate. UBB (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- (TOP) Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with UBB polyclonal antibody (Cat # PAB2409) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. (BUTTOM) Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the UBB polyclonal antibody (Cat # PAB2409) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 7314
- Protein Accession#:
- NP_061828;P62988
- Gene Name:
- UBB
- Gene Alias:
- FLJ25987,MGC8385
- Gene Description:
- ubiquitin B
- Omim ID:
- 191339
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq
- Other Designations:
- OTTHUMP00000064960,OTTHUMP00000064961,polyubiquitin B
- Gene Pathway