非ATP酶蛋白酶体26S亚基7(PSMD7)重组蛋白

YB282Hu013
Recombinant Proteasome 26S Subunit, Non ATPase 7 (PSMD7)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 非ATP酶蛋白酶体26S亚基7(PSMD7)重组蛋白[ PROPERTIES ]
Residues: Met1~Lys324
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P51665
Host: E. coli
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
 非ATP酶蛋白酶体26S亚基7(PSMD7)重组蛋白150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 40.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 非ATP酶蛋白酶体26S亚基7(PSMD7)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
MPELAVQKVV VHPLVLLSVV DHFNRIGKVG NQKRVVGVLL GSWQKKVLDV SNSFAVPFDE
DDKDDSVWFL DHDYLENMYG MFKKVNARER IVGWYHTGPK LHKNDIAINE LMKRYCPNSV
LVIIDVKPKD LGLPTEAYIS VEEVHDDGTP TSKTFEHVTS EIGAEEAEEV GVEHLLRDIK
DTTVGTLSQR ITNQVHGLKG LNSKLLDIRS YLEKVATGKL PINHQIIYQL QDVFNLLPDV
SLQEFVKAFY LKTNDQMVVV YLASLIRSVV ALHNLINNKI ANRDAEKKEG QEKEESKKDR
KEDKEKDKDK EKSDVKKEEK KEKK
[ REFERENCES ]
1. Tsurumi C., et al. (1995) Biochem. Biophys. Res. Commun. 210:600-608. 2. Wang X., et al. (2007) Biochemistry 46:3553-3565. 3. Stelzl U., et al. (2005) Cell 122:957-968. 4. Thompson H.G., et al. (2004) Int. J. Cancer 111:338-347.