人脐静脉内皮细胞说明：
货号： 8000
血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的细胞进行体外研究，人脐静脉和动脉内皮细胞是这类研究中最常用的细胞类型。除了普通的内皮细胞特征例如“鹅卵石状”， VIII因子阳性着色，能摄取乙酰基化低密度脂蛋白，人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。
人脐静脉内皮细胞(HUVEC)提取于人脐带静脉组织，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。 推荐培养基:(ECM, Cat. No. 1001)
Description
The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone. Many of the endothelial processes can be studied in vitro using cultured cells, and human umbilical vein endothelial cells (HUVEC) are the most commonly used cell type for such studies. Except for the common endothelial cell features such as
Recommended Medium
It is recommended to use endothelial cell medium (ECM, Cat. No. 1001) for the culturing of HUVEC in vitro.
Product Use
HUVEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In Human Cell Culture Protocols (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109.
[2] Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247:1219-1222.
[3] Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243:1160-1165.
[4] Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211.
21.脐带细胞系统
8000 人脐静脉内皮细胞(HVVEC)( 5×105 )
8010 人脐带动脉内皮细胞 (HUAEC)( 5×105 )
8020 人脐带静脉平滑肌细胞 (HUVSMC)( 5×105 )
8030 人脐带动脉平滑肌细胞 (HUASMC)( 5×105 )
