Amphiphysin,神经元突触前膜蛋白抗体

产品编号HZ-2409R
英文名称Amphiphysin
中文名称神经元突触前膜蛋白抗体
别 名Amphiphysin; Amp; AMPH 1; AMPH1; Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen); Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); Amphiphysin I; CG8604; Damp; dAmph; Dmel\CG8604; HGNC:471; MGC49429; Stiff man syndrome with breast cancer 128kD autoantigen; Stiff man syndrome with breast cancer 128kDa autoantigen; AMPH_HUMAN.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学
抗体来源Rabbit
Amphiphysin,神经元突触前膜蛋白抗体克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量76kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Amphiphysin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Amphiphysin,神经元突触前膜蛋白抗体PubMedPubMed
产品介绍background:
Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.

Function:
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

Subunit:
Heterodimer with BIN1. Binds SH3GLB1 and AP2A2. Interacts with AP2B1.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton.

Tissue Specificity:
Neurons, certain endocrine cell types and spermatocytes.

Similarity:
Contains 1 BAR domain.
Contains 1 SH3 domain.

Amphiphysin,神经元突触前膜蛋白抗体Gene ID:
273

Database links:
Entrez Gene: 273 Human
Entrez Gene: 218038 Mouse
Entrez Gene: 60668 Rat
Omim: 600418 Human
SwissProt: P49418 Human
SwissProt: Q7TQF7 Mouse
SwissProt: O08838 Rat
Unigene: 592182 Human
Unigene: 101650 Mouse
Unigene: 44463 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Amphiphysin Polyclonal Antibody, Unconjugated(bs-2409R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human meningioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Amphiphysin Polyclonal Antibody, Unconjugated(bs-2409R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining