CRTC1 polyclonal antibody
产品名称： CRTC1 polyclonal antibody
英文名称： CRTC1 polyclonal antibody
产品编号： PAB10265
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of CRTC1.
- Immunogen:
- A synthetic peptide corresponding to amino acids 19-34 of human CRTC1.
- Host:
- Rabbit
- Reactivity:
- Bovine, Human, Mouse, Pufferfishes, Rat, Zebra fish
- Specificity:
- This affinity-purified antibody is directed against human MECT1 protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:10000-1:40000)
Western Blot (1:500-1:20000
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Mect1-Maml2 fusion oncogene linked to the aberrant activation of cyclic AMP/CREB regulated genes.
Coxon A, Rozenblum E, Park YS, Joshi N, Tsurutani J, Dennis PA, Kirsch IR, Kaye FJ.Cancer Res. 2005 Aug 15;65(16):7137-44.
- 2.
- TORCs: transducers of regulated CREB activity.
Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M.Mol Cell. 2003 Aug;12(2):413-23.
- 3.
- t(11;19)(q21;p13) translocation in mucoepidermoid carcinoma creates a novel fusion product that disrupts a Notch signaling pathway.
Tonon G, Modi S, Wu L, Kubo A, Coxon AB, Komiya T, O'Neil K, Stover K, El-Naggar A, Griffin JD, Kirsch IR, Kaye FJ.Nat Genet. 2003 Feb;33(2):208-13. Epub 2003 Jan 21.
- Applications
- Western Blot
- Western blot using CRTC1 polyclonal antibody (Cat # PAB10265) shows detection of CRTC1 (lower arrowhead) and CRTC1 : MAML2 fusion protein (top arrowhead) in cell lysates.
Lane 1 contains lysate from cells expressing CRTC1 only.
Lane 2 contains lysate from cells transfected with fusion protein.
Lane 3 contains lysate from cells strongly expressing CRTC1 and weakly expressing the fusion protein.
Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.
Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.
- Entrez GeneID:
- 23373
- Protein Accession#:
- NP_056136(isoform1);Q6UUV9
- Gene Name:
- CRTC1
- Gene Alias:
- FLJ14027,KIAA0616,MECT1,TORC1,WAMTP1
- Gene Description:
- CREB regulated transcription coactivator 1
- Omim ID:
- 607536
- Gene Ontology:
- Hyperlink
- Other Designations:
- mucoepidermoid carcinoma translocated 1,transducer of regulated cAMP response element-binding protein (CREB) 1