The Blood Direct PCR Kit contains all of the reagents required to rapidly extract and amplify genomic DNA from all kinds of blood samples, like human blood, pig blood, chicken blood, fish blood,frog blood etc. Briefly, the DNA is extracted from 100 ul erythrocyte witho10ut nuclear blood or 1-10 ul erythrocyte with nuclear blood sample incubation in the Extraction Solution at 56°C for 10 minutes, then put it in 95°C for 5 minutes. After an equal volume of the Dilution Solution is added to the extract to neutralize inhibitory substances, the extract is ready for PCR. An aliquot of the diluted extract is then combined with the 2 x Taq Master Mix and user provided PCR primers to amplify target DNA.  2 x Taq Master Mix is a 2 x Reaction Mix containing buffer, salts, dNTPs, and Taq DNA Polymerase. It is optimized specifically for use with the extraction reagents.