肌球蛋白轻链4(MYL4)重组蛋白

YB427Mu011
Myosin Light Chain 4, Alkali, Alkali, Alkali, Alkali, Atrial, Atrial, Atrial, Atrial, Embryonic Embryonic Embryonic Embryonic (MYL4)
Organism: Organism: Organism: Organism: Mus musculus musculus musculus musculus (Mouse) (Mouse) (Mouse) (Mouse)
Instruction Instruction Instruction Instruction manual
 肌球蛋白轻链4(MYL4)重组蛋白NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Lys4~Val187 (Accession # P09541), with
N-terminal His-Tag.
Host: E. coli
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 肌球蛋白轻链4(MYL4)重组蛋白Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.3
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 21.4kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
 肌球蛋白轻链4(MYL4)重组蛋白Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S- KKPEPKK EAAKPAAAPA PAASAAPEPL KDSAFDPKSV KIDFSADQIE
EFKEAFSLFD RTPTGEMKIT YGQCGDVLRA LGQNPTNAEV LRVLGKPKPE EMSSKTLDFE
MFLPILQHIS RNKEQGTYED FVEGLRVFDK ESNGTVMGAE LRHVLATLGE KMSEAEVEQL
LSGQEDANGC INYEAFV