分子量:47.6 kDa

纯度:>97% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human GFR alpha-2, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:MeasuredbyitsbindingabilityinafunctionalELISA. Immobilized Recombinant Human Neurturin at 1 g/mL can bind Human GFR alpha-2, His Tag with an apparent KD < 8 nM.

产品特性:Human GFR alpha-2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.6 kDa. The predicted N-terminus is Ser 22. The reducing (R) protein migrates as 60-70 KDa  in SDS-PAGE  due to glycosylation.

产品背景:GDNF family receptor alpha-2 (GFRA2) is  also known as GDNF receptor beta, Neurturin receptor alpha, RET ligand 2, TGF-beta-related neurotrophic factor receptor 2, is a cell membrane protein which belongs to the GDNFR family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF-beta superfamily of growth factors. Three receptors for these factors, GFRα-1, GFRα-2 and GFRα-3 have been identified. The isoform 1 of GFRA2 is found in both brain and placenta. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor and also able to mediate GDNF signaling through the RET tyrosine kinase receptor. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It can also mediate GDNF signaling through the RET tyrosine kinase receptor.