肝配蛋白B受体3(EPHB3)重组蛋白

YB118Ra012
Recombinant Ephrin Type B Receptor 3 (EPHB3)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
肝配蛋白B受体3(EPHB3)重组蛋白[ PROPERTIES ]
Residues: Val628~Ile891
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P54754
Host: E. coli
Subcellular Location: Cell membrane; Single-pass
type I membrane protein. Cell projection.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
肝配蛋白B受体3(EPHB3)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 33.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
肝配蛋白B受体3(EPHB3)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
VKI EEVIGAGEFG EV CRGR LK LP GRREVFVAIK TLKVGYTERQ RRDFLSEASI
MGQFDHPNII RLEGVVTKSR PVMILTEFME NCALDSFLRL NDGQFTVIQL VGMLRGIAAG
MKYLSEMNYV HRDLAARNIL VNSNLVCKVS DFGLSRFLED DPSDPTYTSS LGGKIPIRWT
AP E AI AY RK F TS A S DVWS Y G IVMWE VMS Y G ERP YW DMS N Q DVI NAV EQ DY
RLPPPMDCPT ALHQLMLDCW VRDRNLRPKF SQIVNTLDKL I