Beta-synuclein,核突触蛋白β/β synuclein抗体

产品编号HZ-6939R
英文名称Beta-synuclein
中文名称核突触蛋白β/β synuclein抗体
别 名beta Synuclein; Phosphoneuroprotein 14; Beta-synuclein; PNP14; SNCB; Synuclein beta; SYUB_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量15kDa
Beta-synuclein,核突触蛋白β/β synuclein抗体细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human beta Synuclein
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Beta-synuclein,核突触蛋白β/β synuclein抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene is highly homologous to alpha-synuclein. These proteins are abundantly expressed in the brain and putatively inhibit phospholipase D2 selectively. The encoded protein, which may play a role in neuronal plasticity, is abundant in neurofibrillary lesions of patients with Alzheimer disease. This protein has been shown to be highly expressed in the substantia nigra of the brain, a region of neuronal degeneration in patients with Parkinson disease; however, no direct relation to Parkinson disease has been established. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].

Function:
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

Subunit:

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed predominantly in brain; concentrated in presynaptic nerve terminals.

DISEASE:

Similarity:
Belongs to the synuclein family.

Gene ID:
6620

Beta-synuclein,核突触蛋白β/β synuclein抗体Database links:
Entrez Gene: 6620 Human
Entrez Gene: 104069 Mouse
Entrez Gene: 113893 Rat
Omim: 602569 Human
SwissProt: Q16143 Human
SwissProt: Q91ZZ3 Mouse
SwissProt: Q63754 Rat
Unigene: 90297 Human
Unigene: 200843 Mouse
Unigene: 20352 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Beta-synuclein/SNCB Polyclonal Antibody, Unconjugated(HZ-6939R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining