SVCT人SV40转化乳腺上皮细胞
产品名称: SVCT人SV40转化乳腺上皮细胞
英文名称: SVCT
产品编号: RE59795
产品价格: 0
产品产地: 中国
品牌商标: 雅吉生物
更新时间: null
使用范围: null
上海雅吉生物科技有限公司
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Synonyms: SVCT人SV40转化乳腺上皮细胞 Background: SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent. Species: human (Homo sapiens) Tissue: breast Disease: Human breast epithelium, SV40 transformed Gender: N/A Morphology: Epithelial Growth Mode: Adherent Doubling Time: N/A DNA Profile: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS
Cell Line Authentication ServiceCulture Medium: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS
We strongly suggest to purchase the complete mediumCryopreservation medium: 90%FBS+10%DMSO Karyotype: Not specified Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.