pREG(R1-R3) #BGV141 
(Plasmid #48958)

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• PURPOSE

  (Empty Backbone) Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
• DEPOSITING LAB

  David Dankort
• PUBLICATION

  Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279.
• PRICE

  $65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

• Vector backbone
  gQxiPuro
  (Search Vector Database)
• Backbone manufacturer
  Dankort lab, unpublished
• Backbone size (bp)7396
• Modifications to backbone
  The R1-R3 Gateway cassette was excised with SacII/HpaI and ligated into gQxiPuro at SacII/EcoRV sites.
• Vector type
  Retroviral
• PromoterCMV

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin and Chloramphenicol
• Growth Temperature
  37°C
• Growth Strain(s)
  ccdB Survival
• Copy number
  Unknown

SEQUENCE INFORMATION

• Depositor Sequences
  Partial (1)
• Addgene Sequences
  Partial (3)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

• Cloning methodGateway Cloning
• 5′ sequencing primerCMV-F
(Common Sequencing Primers)

RESOURCE INFORMATION

• Terms and Licenses
  • UBMTA

 

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

• For your Materials & Methods section:

  pREG(R1-R3) #BGV141 was a gift from David Dankort (Addgene plasmid # 48958)

• For your References section:

  A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D.PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852