谷胱甘肽S转移酶α2(GSTα2)重组蛋白

YB957Mu011
Recombinant Glutathione S Transferase Alpha 2 (GSTa2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
谷胱甘肽S转移酶α2(GSTα2)重组蛋白[ PROPERTIES ]
Residues: Met1~Phe222 (Accession # P09210),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
谷胱甘肽S转移酶α2(GSTα2)重组蛋白sarcosyl and preservative.
Predicted isoelectric point: 8.5
Predicted Molecular Mass: 26.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
谷胱甘肽S转移酶α2(GSTα2)重组蛋白which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MG H HH HH HS GS-MAE KP K L HYS NI R G RMESI R WL LAA AGVE F EEK FIK SAE D
LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKEKA LIDMYIEGIA
DLGEMILLLP FSQPEEQDAK LALIQEKTKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL
VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEESRKIF RF
[ REFERENCES ]
1. Rhoads D.M., et al. (1987) Biochem. Biophys. Res. Commun. 145:474-481. 2. Rohrdanz E., et al. (1992) Arch. Biochem. Biophys. 298:747-752. 3. Klone A., et al. (1992) Biochem. J. 285:925-928. 4. Ahmad H., et al. (1993) Biochim. Biophys. Acta 1161:333-336.