淋巴细胞功能关联抗原2(LFA2)重组蛋白

YB576Ra011
Lymphocyte Lymphocyte Lymphocyte Lymphocyte Function Function Function Function Associated Associated Associated Associated Antigen Antigen Antigen Antigen 2 (LFA2)
Organism: Organism: Organism: Organism: Rattus norvegicus norvegicus norvegicus norvegicus (Rat)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
 淋巴细胞功能关联抗原2(LFA2)重组蛋白Protein Protein Protein Protein Names: Lymphocyte Function Associated
Antigen 2
Synonyms: Synonyms: Synonyms: Synonyms: LFA2, Cd2
Species: Species: Species: Species: Rat
Size: 100µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Membrane; Single-pass type I
 淋巴细胞功能关联抗原2(LFA2)重组蛋白membrane protein.
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Thr27~Leu203 (Accession # P08921), with
N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 23kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 21.7kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 7.1
Rat LFA2
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 淋巴细胞功能关联抗原2(LFA2)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- TVWG ALGHGINLNI PNFQMTDDID EVRWERGSTL VAEFKRKMKP
FLKSGAFEIL ANGDLKIKNL TRDDSGTYNV TVYSTNGTRI LDKALDLRIL EMVSKPMIYW
ECSNATLTCE VLEGTDVELK LYQGKEHLRS LRQKTMSYQW TNLRAPFKCK AVNRVSQESE
MEVVNCPEKG LPL