层粘连蛋白β1(LAMβ1)重组蛋白

YB184Mu011
Laminin Beta 1 (LAMb1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 层粘连蛋白β1(LAMβ1)重组蛋白[ PROPERTIES ]
Residues: Gln1053~Lys1258 (Accession # P07942),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix, basement membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.6
 层粘连蛋白β1(LAMβ1)重组蛋白Predicted Molecular Mass: 54.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 层粘连蛋白β1(LAMβ1)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- QCLCLPNV IGQNCDRCAP
NTWQLASGTG CDP CNCNAAH SFGPS CNEFT GQCQCMPGFG GRTCSECQEL
FWGDP DVE CR ACDCDP RGIE TPQCDQSTG Q CV CVEGVEGP RCDK CT RGYS
GVFPDCTPCH QCFALWDVII AELTNRTHRF LEKAKALKIS GVIGPYRETV DSVERKVSEI
KDILAQSPAA EPLKNIGNLF EEAEKLIK
[ REFERENCES ]
1. Wu Y., et al. (2011) Int. J. Neurosci. 121:323-328. 2. Taniguchi Y., et al. (2009) J. Biol. Chem. 284:7820-7831. 3. Fujita M., et al. (2005) Breast Cancer Res. 7:R411-21. 4. Jaye M., et al. (1987) Am. J. Hum. Genet. 41:605-615.