FITC标记的磷酸化活化复制因子2抗体
产品名称： FITC标记的磷酸化活化复制因子2抗体
英文名称： Anti-phospho-ATF2 (Thr71 + Thr53)/FITC
产品编号： HZ-12541R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-phospho-ATF2 (Thr71 + Thr53)/FITC Conjugated antibody
FITC标记的磷酸化活化复制因子2抗体
| 英文名称 | Anti-phospho-ATF2 (Thr71 + Thr53)/FITC |
| 中文名称 | FITC标记的磷酸化活化复制因子2抗体 |
| 别 名 | ATF2 (phospho T71 + T53); p-ATF2 (phospho T71 + T53); ATF2 (phospho T71/T53); ATF2 (phospho Thr71/Thr53); CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Thr71 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain. Function: Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. Subunit: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Subcellular Location: Nucleus. Tissue Specificity: Abundant expression seen in the brain. Post-translational modifications: Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger. Database links: Entrez Gene: 1386 Human Entrez Gene: 100047997 Mouse Entrez Gene: 11909 Mouse Entrez Gene: 81647 Rat Omim: 123811 Human SwissProt: P15336 Human SwissProt: P16951 Mouse SwissProt: Q00969 Rat Unigene: 592510 Human Unigene: 209903 Mouse Unigene: 9825 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
ATF2是碱性区域亮氨酸拉链DNA结合蛋白ATF/CREB家族的成员，该蛋白通过与多种病毒和细胞基因启动子中一致的cAMP反应元件（CRE）结合来调节转录。这些基因中的许多在细胞生长和分化、应激和免疫应答中都是重要的。ATF2是一种核蛋白，结合DNA作为二聚体，并能与ATF/CREB和JU/FOS家族成员形成二聚体。它是一个较强的激活剂作为异丙醇与CJun比作为同源二聚体。ATF2的几种异构体由差异拼接产生。稳定的天然全长ATF2由于其羧基末端DNA结合域与氨基末端反式激活域的抑制性直接分子内相互作用而转录失活。在二聚之后，ATF2变成一个短命的蛋白质，经历泛素化和蛋白水解，似乎是在蛋白磷酸酶依赖的机制中。ATF2转录活性的刺激发生在多种遗传毒性物质、炎性细胞因子和紫外线诱导的细胞应激之后。这种活化需要由JNK/SAP激酶和p38MAP激酶在ATF2中磷酸化两个苏氨酸残基。ATF2在脑内大量表达。