特异性蛋白1(Sp1)重组蛋白

YB264Hu011
Recombinant Specificity Protein 1 (Sp1)
Organism : Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
特异性蛋白1(Sp1)重组蛋白NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Leu260~Ala591 (Accession # P08047),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Nucleus. Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
特异性蛋白1(Sp1)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and preservative.
Predicted isoelectric point: 5.5
Predicted Molecular Mass: 65.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
特异性蛋白1(Sp1)重组蛋白KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- L NGNITLLPVN SVSAATLTPS
SQAVTISSSG SQESGSQPVT SGTTISSASL VSSQASSSSF FTNANSYSTT TTTSNMGIMN
FT TSGSSGT N SQ GQTPQRVS GLQGS DA LNI QQNQTSGGS L QAGQQKEGEQ
NQQTQQQQIL IQPQLVQGGQ ALQALQAAPL SGQTFTTQAI SQETLQNLQL QAVPNSGPII
IRTPTVGPNG QVSWQTLQLQ NLQVQNPQAQ TITLAPMQGV SLGQTSSSNT TLTPIASAAS
IPAGTVTVNA AQLSSMPGLQ TINLSALGTS GIQVHPIQGL PLAIANAPGD HGAQLGLHGA
GGDGIHDDTA GGEEGENSPD A
[ REFERENCES ]
1. Infantino V., et al. (2011) Biochem. Biophys. Res. Commun. 412:86-91. 2. Takahara T., et al. (2000) J. Biol. Chem. 275:38067-38072. 3. Jackson S.P., Tjian R. (1988) Cell 55:125-133. 4. Courey A.J., Tjian R. (1988) Cell 55:887-898.