HpaI-核酸/蛋白合成-试剂-生物在线

产品名称： HpaI

英文名称： HpaI

产品编号： HZ600188

产品价格： 0

产品产地：中国/上海

品牌商标： HZbscience

更新时间： 2023-08-17T10:24:20

使用范围： null

上海沪震实业有限公司

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HpaI

产品描述

使用说明

高浓度results in星的酶活性。

技术规格

Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时37°C in at总量在50μl反应。

网站gtt↑aac recognition

大肠杆菌来源的应变检测HPA基因，that from the带一副流感嗜血杆菌

我们assayedλDNA

优化和缓冲区？（33毫米的Tris乙酸（pH 7.9毫米（10°C）；醋酸醋酸钾镁；66毫米；1毫米DTT。）

37°C温度的优化

10毫米的存储条件的Tris-HCl（pH 7.5）；50 mM NaCl 0.1毫米1毫米；EDTA；BSA DTT；200μg／ml；50%的甘油。商店在零下20摄氏度。

ligations after about with酶的5倍overdigestion 60% of the DNA碎片重新ligated and can be。

非特异性水解后没有检测到孵化nonspecific activity was withλDNA 1μg of of of 5 16 hours for UA听说是八周的酶在37°C。

甲基化效应不灵敏的测试

热灭活的灭活酶是由孵化在150度为20分钟。

在campatible bsthpi，kspai

产品描述

使用说明

High enzyme concentration results in star activity.

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Recognition sites	GTT↑AAC
Source	E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae
Assayed on	Lambda DNA
Optimal buffer	Y?(33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature	37°C
Storage conditions	10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations	After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65°C for 20 mins.
Campatible Ends	BstHPI, KspAI