StemSpan™ CD34+ Expansion Supplement (10X) contains a combination of recombinant human cytokines (SCF, IL-3, IL-6 and TPO) and other additives formulated to selectively support the expansion of CD34+ human cord blood (CB) and bone marrow (BM) cells in liquid culture. It is supplied as a 10X concentrate and intended for use in combination with StemSpan™ SFEM, SFEM II and ACF serum-free expansion media, or any other media for culturing human hematopoietic cells.

StemSpan™ CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human CB cells. After one week, ~40% of the cultured cells express CD34, indicative of >10-fold expansion of input CD34+ CB cells. See data tab for more details.




Application: Expansion
Product Type: Specialized cell culture media
Recommended for: •Expansion of human hematopoietic stem and progenitor cells in culture, characterized by their expression of the CD34 antigen
•Induction of stem and immature progenitor cell cycling for studies of signal transduction and metabolism, or to facilitate retroviral gene transfer
•“Pre-amplification” of hematopoietic progenitor cells to increase the yield of mature cells in lineage-specific expansion and differentiation cultures
•Assessment of efficacy and toxicity of candidate therapeutics on human hematopoietic stem and progenitor cells for drug development
Accessory Products: StemSpan™ SFEM (Catalog #09600/09650)
StemSpan™ SFEM II (Catalog #09605/09655)
StemSpan™-ACF (Catalog #09805/09855)
Contains: • rh Stem Cell Factor
• rh Thrombopoietin
• rh Interleukin-3
• rh Interleukin-6
• rh Flt-3 Ligand
• Other additives
Advantages: •Formulated to selectively expand and produce large numbers of human CD34+ hematopoietic cells in liquid cultures initiated with CD34+ CB or BM cells.
•Optimized for use with StemSpan™ media. When combined with StemSpan™ SFEM II in particular, supports at least 50% higher expansion of CD34+ human CB cells when compared to other serum-free media on the market.
•Supplied as a 10X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium of choice.
Area of Interest: Hematopoietic stem cell research, Stem cell biology
Cell Type: Hematopoietic stem & progenitor cells
Medium Type: Liquid, Serum-free
Popular Product Line: StemSpan™
Species: Human
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.