Anti-CRISPR-Cas9抗体
产品名称： Anti-CRISPR-Cas9抗体
英文名称： CRISPR-Cas9
产品编号： YB--52042M
产品价格： null
产品产地： 中国/美国
品牌商标： Ybscience
更新时间： 2023-08-17T10:29:50
使用范围： 科研使用
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Anti-CRISPR-Cas9抗体
| 产品编号 | YB-52042M |
| 英文名称 | CRISPR-Cas9 |
| 中文名称 | CRISPR-Cas9小鼠单克隆抗体 |
| 别 名 | Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; CAS9_STRP1. 酿脓链球菌 |
| 规格价格 | 50ul/1580元 购买 100ul/2680元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul |
| 研究领域 | |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 1E8 |
| 交叉反应 | Streptococcus pyogenes |
| 产品应用 | WB=1:500-2000 ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 160kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (N terminal): |
| 亚 型 | IgM |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence. Function: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself. Subunit: Monomer. Binds crRNA and tracrRNA. Similarity: Belongs to the CRISPR-associated protein Cas9 family. Subtype II-A subfamily. SWISS: Q99ZW2 Gene ID: 901176 Database links: Entrez Gene: 901176 Streptococcus pyogenes SwissProt: Q99ZW2 Streptococcus pyogenes Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |