GST MULTITRAP 4B
产品名称： GST MULTITRAP 4B
英文名称： GST MULTITRAP 4B
产品编号： 28-4055-00
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
- 传真 : 点击查看
- 邮箱 : 342786402@qq.com
| 28-4055-00 | GST MULTITRAP 4B |
| 28-4055-01 | GST MULTITRAP FF |
| 28-9523-59 | GST SpinTrap |
| 28-9523-60 | GST GraviTrap |
| 28-9523-61 | GST Buffer Kit |
| 28-9545-49 | PGEX-4T-1 |
| 28-9545-50 | PGEX-4T-2 |
| 28-9545-52 | PGEX-4T-3 |
| 28-9545-53 | PGEX-5X-1 |
| 28-9545-54 | PGEX-5X-2 |
| 28-9545-55 | PGEX-5X-3 |
| 28-9546-46 | PGEX-2TK |
| 28-9546-48 | PGEX-6P-1 VECTOR |
| 28-9546-50 | PGEX-6P-2 VECTOR |
| 28-9546-51 | PGEX-6P-3 VECTOR |
| 28-9546-53 | PGEX-2T 25 UG WI/DI |
| 28-9546-54 | PGEX-3X 25 UG WI/DI |
| 28-9546-56 | PGEX-1LT ECOR I/BAP |
| 11-0033-99 | HIS GRAVITRAP |
| 11-0034-00 | HIS BUFFER KIT |
| 27-4710-01 | ANTI-HIS ANTIBODY |
| 28-4009-89 | HIS MULTITRAP HP |
| 28-4009-90 | HIS MULTITRAP FF |
| 28-4013-53 | HIS SPINTRAP |
| 28-4039-43 | COLLECTION PLATE |
| 28-9321-71 | His SpinTrap Kit |
| 27-1583-01 | LIGHT PRIMER MIX |
| 27-1586-01 | HEAVY PRIMER MIX |
| 27-1588-01 | LINKER-PRIMER MIX |
| 27-1589-01 | RS PRIMER MIX |
| 27-9420-01 | ANTI-M13 MONOCLONAL ANTIBODY |
| 27-9421-01 | HRP/ANTI-M13 MONOCLNL CONJUG. |
GST MultiTrap 4B
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione SepharoseTM media for convenience.
- Easy and predictable scale-up using prepacked GSTrapTM FF, GSTPrepTM FF 16/10, and GSTrapTM 4B columns.
GST MultiTrapTM FF and GST MultiTrapTM 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.
Purification of up to 0.5 mg of GST-tagged proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrapTM filter plates. Direct purification of GST-tagged proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
GST MultiTrapTM FF is prepacked with Glutathione SepharoseTM 4 fast flow, while GST MultiTrapTM 4B is prepacked with Glutathione SepharoseTM 4B.
GST MultiTrap 4B — Technical Specifications
| Complete Packsize | 4 x 96-well filter plates |
| Storage Conditions | 4 - 30°C, 20% Ethanol |
| Centrifugation Force Recommended | 100 x g-500 x g |
| Centrifugation Force Max. | 700 x g |
| Media | |
|---|---|
| Ligand | Glutathione |
| Average Particle Size | 90 μm |
| Matrix | Agarose, 4% |
| Particle Size | 45 µm-165 µm |
| Binding Capacity/ml Chromatography Medium | > 25 mg horse liver GST/ml medium1) |
| pH stability Working Range | 4-13 |
| pH stability Cleaning | 4-13 |
| Ligand Coupling Method | Epoxy activation |
| Storage Conditions | 4 to 30°C, 20% Ethanol |
| Chemical Stability | All commonly used aqueous buffers |
| 1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| Column | |
|---|---|
| Complete Packsize | 4 x 96-well filter plates |
| Number of Wells | 96 |
| Sample Volume | 600 μl/well |
| Reproducibility Between Wells | ± 10% in yield |
| Material | Polypropylene (PP) and polyethylene (PE). |
| Dimensions | 127.8 x 85.5 x 30.6 mm |
|
Licensing |
For licensing information, see the Licensing Statements page in the About Us section |