大鼠生长分化因子11(GDF11)多克隆抗体
Polyclonal Antibody to Growth Differentiation Factor 11 (GDF11)
[PRODUCT INFORMATION]
Immunogen: GDF11, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant GDF11 (Cys105~Pro341) expressed in E.coli. 
Sequence: The target protein is fused with two N-terminal Tags, His-tag andT7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CCHFHF SPKVMFTKVLKAQLWVYLRP VPRPATVYLQ ILRLKPLTGE GTAGGGGGGR RHIRIRSLKI ELHSRSGHWQSIDFKQVLHS WFRQPQSNWG IEINAFDPSG TDLAVTSLGP GAEGCHPFME LRVLENTKRSRR N L G L D C DE HS S E S R C C RY P LT V D F E A S G WDWII A P K RY KA NY CSG Q CEYMFMQKYPHT HLVQQANPRG SAGPCCTPTK MSPINMLYFN DKQQIIYGKI P
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against GDF11. It has beenselected for its ability to recognize GDF11 in immunohistochemical staining andwestern blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant GDF11 (Cys105~Pro341)disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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