DRAM,DNA损伤自噬调整蛋白抗体

产品编号HZ-10285R
英文名称DRAM
中文名称DNA损伤自噬调整蛋白抗体
别 名Damage regulated autophagy modulator; Damage-regulated autophagy modulator; DNA damage-regulated autophagy modulator protein 1; dram1; DRAM1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 心血管 免疫学 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep,
DRAM,DNA损伤自噬调整蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量26kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DRAM
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DRAM,DNA损伤自噬调整蛋白抗体PubMedPubMed
产品介绍background:
This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. [provided by RefSeq, Jul 2008]

Function:
Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.

Subcellular Location:
Lysosome membrane; Multi-pass membrane protein.

Similarity:
Belongs to the DRAM/TMEM150 family.

Gene ID:
55332

Database links:
Entrez Gene: 55332 Human
Entrez Gene: 71712 Mouse
Omim: 610776 Human
SwissProt: Q8N682 Human
SwissProt: Q9DC58 Mouse
Unigene: 525634 Human
Unigene: 35450 Mouse

DRAM,DNA损伤自噬调整蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Protein: k562 lysate;  Primary: rabbit Anti-DRAM (HZ-10285R) at 1:300;  Secondary: HRP conjugated Goat-Anti-rabbit IgG(HZ-0295G-HRP) at 1: 5000;  Predicted band size: 26 kD Observed band size: 26 kD Tissue/cell: human skin squamous carcinoma;  Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-DRAM Polyclonal Antibody, Unconjugated(HZ-10285R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining