乙酰化组蛋白3（K14）抗体
产品名称： 乙酰化组蛋白3（K14）抗体
英文名称： Histone H3 (acetyl K14)
产品编号： hz-23417R
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
Rabbit Anti-Histone H3 (acetyl K14) antibody
| 产品编号 | hz-23417R |
| 英文名称 | Histone H3 (acetyl K14) |
| 中文名称 | 乙酰化组蛋白3（K14）抗体 |
| 别 名 | Histone H3 (Lys14) ; H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H31_HUMAN |
| 产品类型 | 乙酰化抗体 |
| 研究领域 | 肿瘤 细胞生物 发育生物学 染色质和核信号 信号转导 通道蛋白 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Histone H3 (acetyl K14) :GG(Acetyl-K)AP |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling. Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination. Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases histone-protein interactions. Interacts with PDD1 and PDD3. Subcellular Location: Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC). Post-translational modifications: Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or meiotic micronuclear divisions. Acetylation of histone H3 leads to transcriptional activation. H3K14ac formation by GCN5 is promoted by H3S10ph. H3K9acK14ac is the preferred acetylated form of newly synthesized H3. Acetylation occurs almost exclusively in the MAC. Methylated to form H3K4me. H3K4me is only found in the transcriptionally active MAC. Methylated to form H3K9me in developing MACs during conjugation, when genome-wide DNA elimination occurs. At this stage, H3K9me specifically occurs on DNA sequences being eliminated (IES), probably targeted by small scan RNAs (scnRNAs) bound to IES, and is required for efficient IES elimination. H3K9me is required for the interaction with the chromodomains of PDD1 and PDD3. The full-length protein H3S (slow migrating) is converted to H3F (fast migrating) by proteolytic removal of the first 6 residues. H3F is unique to MIC, and processing seems to occur regularly each generation at a specific point in the cell cycle. Similarity: Belongs to the histone H3 family. SWISS: P68431 Gene ID: 8290 Database links:
Entrez Gene: 8290 Human Entrez Gene: 8350 Human Entrez Gene: 8351 Human Entrez Gene: 8352 Human Entrez Gene: 8353 Human Entrez Gene: 8354 Human Entrez Gene: 8355 Human Entrez Gene: 8356 Human Entrez Gene: 8357 Human Entrez Gene: 8358 Human Entrez Gene: 8968 Human Entrez Gene: 260423 Mouse Entrez Gene: 319148 Mouse Entrez Gene: 319149 Mouse Entrez Gene: 319150 Mouse Entrez Gene: 319151 Mouse Entrez Gene: 319152 Mouse Entrez Gene: 319153 Mouse Entrez Gene: 360198 Mouse Entrez Gene: 97908 Mouse Entrez Gene: 100364501 Rat Entrez Gene: 100365669 Rat Entrez Gene: 291159 Rat Entrez Gene: 314977 Rat Entrez Gene: 364716 Rat Entrez Gene: 679950 Rat Entrez Gene: 679994 Rat Entrez Gene: 680511 Rat Entrez Gene: 680599 Rat Entrez Gene: 682330 Rat Entrez Gene: 691496 Rat Omim: 601128 Human Omim: 602810 Human Omim: 602811 Human Omim: 602812 Human Omim: 602813 Human Omim: 602814 Human Omim: 602815 Human Omim: 602816 Human Omim: 602817 Human Omim: 602818 Human Omim: 602819 Human SwissProt: P68431 Human SwissProt: P84243 Human SwissProt: Q16695 Human SwissProt: Q6NXT2 Human SwissProt: Q71DI3 Human SwissProt: P68433 Mouse SwissProt: P84228 Mouse SwissProt: Q6LED0 Rat TUNEology 波长可调检测卡盒--> |