PRODUCT DATA SHEET
KAMIYA BIOMEDICAL COMPANY, Seattle, WA USA Tel: (206) 575-8068 • Fax: (206) 575-8094
www.kamiyabiomedical.com techsupport@kamiyabiomedical.com
Product: Anti-IKKa (C1), Polyclonal
Cat. No: PC-040 (100 μg)
Background:
Nuclear factor kappa b (NF-kb) is a ubiquitous
transcription factor and an essential mediator of
gene expression during activation of immune
and inflammatory responses. NF-kb mediates
the expression of a great variety of genes in
response to extracellular stimuli including IL-1,
TNFa and bacteria product LPS. NF-kB is
associated with IkB proteins in the cell
cytoplasm, which inhibit NF-kb activity. The
long-sought Ikb kinase (IKK), which phosphorylates
kb, and mediates IkB degradation
and NF-kB activation, was recently identified by
several laboratories. IKK is a serine protein
kinase, and the IKK complex contains alpha and
beta subunits (IKKa and IKKb). IKKa and IKKb
interact with each other and both are essential
for the NF-kB activation. IKKa specifically
phosphorylates Ikb-a. IKKa is expressed in
variety of human tissues.
Specificity:
Binds human IKKa. It has no cross response to
IKKb or IKKg
Positive Control: Whole cell lysate from HeLa or
Jurkat cells. An 85 kDa band should be
detected by Western blot.
Species Reactivity:
Human, other species not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
A peptide corresponding to amino acids 716 to
734 of human IKKa, which differs from the
corresponding murine sequence by four amino
acids.
Format:
Immunoaffinity chromatography purified IgG,
100 μg in 200 μl of PBS containing 0.02
sodium azide.
Storage and Stability:
Stable for at least one year when stored at +4°C.
Applications and Suggested Dilutions:

Western blot: Suggest 1:1000 to 1:2000.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product