N-乙酰α-D-氨基葡萄糖苷酶(NAGLU)重组蛋白

YB161Ra011
Recombinant N-Acetyl Alpha-D-Glucosaminidase (NAGLU)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
N-乙酰α-D-氨基葡萄糖苷酶(NAGLU)重组蛋白[ PROPERTIES ]
Residues: Val485~Trp743 (Accession # P54802),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Lysosome.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.8
N-乙酰α-D-氨基葡萄糖苷酶(NAGLU)重组蛋白Predicted Molecular Mass: 33.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VSHPDA GAAWRLLLRS
VYNCSGEACR GHNRSPLVRR PSLQMNTSIW YNRSDVFEAW RLLLTSAPSL ATSPAFRYDL
N-乙酰α-D-氨基葡萄糖苷酶(NAGLU)重组蛋白LDLTRQAVQE LVSLYYEEAR SAYLSKELAS LLRAGGVLAY ELLPALDEVL ASDSRFLLGS
WLEQARAAAV SEAEADFYEQ NSRYQLTLWG PEGNILDYAN KQLAGLVANY YTPRWRLFLE
ALVDSVAQGI PFQQHQFDKN VFQLEQAFVL SKQRYPSQPR GDTVDLAKKI FLKYYPRWVA
GSW
[ REFERENCES ]
1. Zhao H.G., et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93:6101-6105. 2. Weber B., et al. (1996) Hum. Mol. Genet. 5:771-777. 3. Zhao Z., et al. (1996) Mamm. Genome 7:686-690. 4. Chen R., et al. (2009) J. Proteome Res. 8:651-661.