烟碱型胆碱受体α4(CHRNα4)重组蛋白

YB913Mu013
Recombinant Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 烟碱型胆碱受体α4(CHRNα4)重组蛋白[ PROPERTIES ]
Residues: Ile32~Thr249
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O70174
Host: E. coli
Subcellular Location: Cell junction, synapse,
postsynaptic cell membrane. Multi-pass membrane
protein. Cell membrane. Lipid-anchor. Purity: >90%
Endotoxin Level: <1.0EU per 1μg
 烟碱型胆碱受体α4(CHRNα4)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 29.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 烟碱型胆碱受体α4(CHRNα4)重组蛋白he sequence of the target protein is listed below.
IETRAHAEE RLLKRLFSGY NKWSRPVANI SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW
VKQEWHDYKL RWDPGDYENV TSIRIPSELI WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR
VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK FGSWTYDKAK IDLVSMHSRV DQLDFWESGE
WVIVDAVGTY NTRKYECCAE IYPDITYAFI IRRLPLFYT