羧肽酶A2(CPA2)重组蛋白

YB316Ra011
Recombinant Carboxypeptidase A2, Pancreatic (CPA2)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 羧肽酶A2(CPA2)重组蛋白[ PROPERTIES ]
Residues: Leu26~Tyr417
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P19222
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
 羧肽酶A2(CPA2)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.2
Predicted Molecular Mass: 47.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
 羧肽酶A2(CPA2)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
LEIIP SHEEQIRTLL QLEAEEHLEL DFWKSPTIPG ETVHVRVPFA SIQAVKVFLE SQGIDYSIMI
EDVQVLLDQE REEMLFNQQR ERGGNFNFEA YHTLEEIYQE MDNLVAENPG LVSKVNLGSS
FENRPMNVLK FSTGGDKPAI WLDAGIHARE WVTQATALWT ANKIASDYGT DPAITSLLNT
LDI F L LP VT N PDGY VF SQ T T NRMW RKT RSK RSGS G CV GV D PN RNW DA N FG
GPGASSSPCS DSYHGPKPNS EVEVKSIVDF IKSHGKVKAF ITLHSYSQLL MFPYGYKCTK
PDDFNELDEV AQKAAQALKR LHGTSYKVGP ICSVIYQASG GSIDWAYDLG IKYSFAFELR
DTAFYGFLLP AKQILPTAEE TWLGLKTIME HVRDHPY