核仁素(NCL)重组蛋白

YB242Mu012
Recombinant Nucleolin (NCL)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
12th Edition (Revised in Aug, 2016)
 核仁素(NCL)重组蛋白 PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Lys388~Gln569
Tags: N-terminal His-Tag
Subcellular Location: Nucleolus. Cytoplasm. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
 核仁素(NCL)重组蛋白Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 24.3kDa
Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
 核仁素(NCL)重组蛋白oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE