CAPG抗体
Applications
In-house applications |
ELISA, WB, IHC, IF, IP Application not tested? Please see our Guarantee. |
Published applications |
IHC, WB |
Publications |
10 citations –view here |
Species specificity |
Human,Mouse,Rat; other species not tested. |
Published Species |
Human, mouse |
Images
Prev12Next
- HeLa cells were subjected to SDS PAGE followed by western blot with 10194-1-AP(CAPG antibody) at dilution of 1:1000
- Immunohistochemical of paraffin-embedded human prostate cancer using 10194-1-AP(CAPG antibody) at dilution of 1:100 (under 10x lens)
- Immunofluorescent analysis of MCF-7 cells, using CAPG antibody 10194-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- IP Result of anti-CAPG (IP:10194-1-AP, 4ug; Detection:10194-1-AP 1:500) with mouse heart tissue lysate 4000ug.
|
Positive WB detected in |
HeLa cells, HL-60 cells, mouse heart tissue, mouse thymus tissue, rat heart tissue, rat lung tissue |
Positive IP detected in |
Mouse heart tissue |
Positive IHC detected in |
Human prostate cancer tissue, human tonsillitis tissue |
Positive IF detected in |
MCF-7 cells |
Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200 |
CAPG抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source |
Rabbit |
Purification method |
Antigen affinity purification |
Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
Immunogen |
CAPG fusion protein ag0248 |
Full name |
Capping protein (actin filament), gelsolin-like |
Calculated molecular
weight |
39 kDa |
Observed molecular
weight |
39-42kd |
GenBank accession
number |
BC000728 |
Gene ID (NCBI) |
822 |
Gene symbol |
CAPG |
Synonyms |
Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP |
Background CAPG抗体
CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129) CAPG抗体
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