骨成型蛋白4(BMP4)重组蛋白

YB014Rb031
Bone Morphogenetic Protein 4 (BMP4)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 骨成型蛋白4(BMP4)重组蛋白[ PROPERTIES ]
Residues: Ser219~Lys392
Tags: N-terminal His-Tag
Accession: Q2KJH1
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
 骨成型蛋白4(BMP4)重组蛋白sarcosyl and preservative.
Predicted isoelectric point: 9.7
Predicted Molecular Mass: 21.4kDa
Accurate Molecular Mass: 16&18&24kDa as determined by SDS-PAGE reducing
conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
 骨成型蛋白4(BMP4)重组蛋白SP AVLRWTREKQ PNYGLAIEVT HLHQTRTHQG QHVRISRSLP QGSGDWAQLR
P L LV T F G H D G RG HA LT R R R R AK RS P K H HP Q RA RK K N K N C R RHS LY V D F S D
VGWNDWIVAP PGYQAFYCHG DCPFPLADHL NSTNHAIVQT LVNSVNSSIP KACCVPTELS
AISMLYLDEY DK
[ REFERENCES ]
1. Knöchel S., et al. (2001) Mech. Dev. 109 (1): 79–82.
2. Nilsson E., Skinner M. (2003) Biol. Reprod. 69 (4): 1265–72.
3. Arteaga-Solis E., et al. (2001) J. Cell Biol. 154 (2): 275–81.
4. Miyazaki Y., et al. (2003) Kidney Int. 63 (3): 835–44.