pOZ-FH-C-puro
(Plasmid #32516)

PURPOSE

(Empty Backbone)
DEPOSITING LAB

Andrew Lassar
PUBLICATION

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. Epub 2009 May 20.
PRICE

$65 (USD)

Add to Cart

Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
pOZ-FH-C
(Search Vector Database)
Backbone manufacturer
Created by Yoshihiro Nakatani
Backbone size (bp)7900
Modifications to backbone
pOZ-FH-C-puro vector was made by modifying the pOZ-FH-C vector (Nakatani and Ogryzko, 2003). A PCR-amplified puromycin resistance gene (isolated from pBabe-puro) was cloned in place of interleukin-2Rα using NcoI and BamHI site.
Vector type
Mammalian Expression, Retroviral
Selectable markers
Puromycin
Tag / Fusion Protein
- FLAG and HA (C terminal on backbone)

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

RESOURCE INFORMATION

Terms and Licenses
- UBMTA

DEPOSITOR COMMENTS

pOZ-FH-C-puro vector was made by modifying the pOZ-FH-C vector (Nakatani and Ogryzko, 2003). A PCR-amplified puromycin resistance gene (isolated from pBabe-puro) was cloned in place of interleukin-2Rα using NcoI and BamHI site.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pOZ-FH-C-puro was a gift from Andrew Lassar (Addgene plasmid # 32516)
For your References section:

Id3 is a direct transcriptional target of Pax7 in quiescent satellite cells. Kumar D, Shadrach JL, Wagers AJ, Lassar AB. Mol Biol Cell. 2009 Jul;20(14):3170-7. Epub 2009 May 20. 10.1091/mbc.E08-12-1185 PubMed 19458195

pOZ-FH-C-puro 逆病毒载体质粒图谱序列抗性

pOZ-FH-C-puro (Plasmid #32516)