3-琥珀酰辅酶A转移酶1(OXCT1)重组蛋白

YB480Hu015
Recombinant 3-Oxoacid Coenzyme A Transferase 1 (OXCT1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 3-琥珀酰辅酶A转移酶1(OXCT1)重组蛋白[ PROPERTIES ]
Residues: Thr40~Leu489
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P55809
Host: E. coli
Subcellular Location: Mitochondrion matrix.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
 3-琥珀酰辅酶A转移酶1(OXCT1)重组蛋白Predicted isoelectric point: 6.3
Predicted Molecular Mass: 52.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 3-琥珀酰辅酶A转移酶1(OXCT1)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
T KFYTDPVEAV KDIPDGATVL VGGFGLCGIP ENLIDALLKT GVKGLTAVSN NAGVDNFGLG
LLLRSKQIKR MVSSYVGENA EFERQYLSGE LEVELTPQGT LAERIRAGGA GVPAFYTPTG
YGTLVQEGGS PIKYNKDGSV AIASKPREVR EFNGQHFILE EAITGDFALV KAWKADRAGN
VIFRKSARNF NLPMCKAAET TVVEVEEIVD IGAFAPEDIH IPQIYVHRLI KGEKYEKRIE
RLSIRKEGDG EAKSAKPGDD VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNITVH
LQSENGVLGL GPYPRQHEAD ADLINAGKET VTILPGASFF SSDESFAMIR GGHVDLTMLG
AMQVSKYG D L ANWMIPGKMV KGMGGAMD LV SSAKTKVVVT ME HSAKGNA H
KIMEKCTLPL TGKQCVNRII TEKAVFDVDK KKGLTLIEL