端粒酶逆转录酶(TERT)重组蛋白

YB241Ra012
Recombinant Telomerase Reverse Transcriptase (TERT)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 端粒酶逆转录酶(TERT)重组蛋白[ PROPERTIES ]
Residues: Arg787~Arg1084
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: O14746
Host: E. coli
Subcellular Location: Nucleus; Chromosome.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 端粒酶逆转录酶(TERT)重组蛋白Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 65.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
 端粒酶逆转录酶(TERT)重组蛋白Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
RDAV VIEQSSSLNE ASSGLFDVFL RFMCHHAVRI RGKSYVQCQG IPQGSILSTL
LCSLCYGDME NKLFAGIRRD GLLLRLVDDF LLVTPHLTHA KTFLRTLVRG VPEYGCVVNL
RKTVVNFPVE DEALGGTAFV QMPAHGLFPW CGLLLDTRTL EVQSDYSSYA RTSIRASLTF
NRGFKAGRNM RRKLFGVLRL KCHSLFLDLQ VNSLQTVCTN IYKILLLQAY RFHACVLQLP
FHQQVWKNPT FFLRVISDTA SLCYSILKAK NAGMSLGAKG AAGPLPSEAV QWLCHQAFLL
KLTR
[ REFERENCES ]
1. Meyerson M., et al. (1997) Cell 90:785-795. 2. Wick M., et al. (1999) Gene 232:97-106. 3. Hisatomi H., et al. (2003) Neoplasia 5:193-197. 4. Nagao K., et al. (2004) Oncol. Rep. 11:127-131.