ALPL Validated Chimera RNAi-RNAi技术-试剂-生物在线

产品名称： ALPL Validated Chimera RNAi

英文名称： ALPL Validated Chimera RNAi

产品编号： H00000249-R01V

产品价格： 0

产品产地：台湾

品牌商标： Abnova

更新时间： null

使用范围： null

亚诺法生技股份有限公司（Abnova)

进入展台

Storage Instruction:
Store at -20°C, do not exceed 4 - 5 freeze-thaw cycles to ensure product integrity.

1.
dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.
Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91.

3.
Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.
Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K.Nucleic Acids Res. 2004 Feb 9;32(3):936-48. Print 2004.

4.
siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference.
Naito Y, Yamada T, Ui-Tei K, Morishita S, Saigo K.Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W124-9.

RNAi Knockdown (Antibody validated)
Western blot analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Protocol Download

Gene Summary:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq

Other Designations:
OTTHUMP00000002971,OTTHUMP00000002972,alkaline phosphatase, tissue-nonspecific isozyme,alkaline phosphomonoesterase,glycerophosphatase,liver/bone/kidney-type alkaline phosphatase,tissue non-specific alkaline phosphatase,tissue-nonspecific ALP