ARIH2,E3连接酶蛋白ARIH2抗体

产品编号HZ-12521R
英文名称ARIH2
中文名称E3连接酶蛋白ARIH2抗体
别 名all trans retinoic acid inducible RING finger; Ari 2; Ari-2; ARI2; ARI2_HUMAN; Ariadne 2; Ariadne 2 protein homolog; ariadne homolog 2 (Drosophila); ariadne homolog 2; ARIH 2; Arih2; E3 ubiquitin protein ligase ARIH2; E3 ubiquitin-protein ligase ARIH2; HT005; protein ariadne 2 homolog; Protein ariadne-2 homolog; Triad 1; TRIAD1; Triad1 protein; UIP48.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 信号转导 表观遗传学 泛素
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit,
ARIH2,E3连接酶蛋白ARIH2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量58kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ARIH2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ARIH2,E3连接酶蛋白ARIH2抗体PubMedPubMed
产品介绍background:
TRIAD1, also known as ARIH2 (ariadne homolog 2) or ARI2, is a 493 amino acid protein that contains one IBR-type zinc finger and two RING-type zinc fingers and belongs to the ariadne subfamily of RBR proteins. Localized to the nucleus, TRIAD1 interacts with UBE2L3 and is thought to act as an E3 ubiquitin-protein ligase, functioning to accept ubiquitin from E2 ubiquitin-conjugating enzymes and transfer the acquired ubiquitin residue to target substrates. TRIAD1 is subject to post-translational DNA damage-dependent phosphorylation, probably by ATM or ATR. The gene encoding TRIAD1 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.

Function:
E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.

Subunit:
Interacts (via RING-type 1) with UBE2L3. Interacts (via RING-type 2) with UBE2N. Interacts (via RING-type 2) with GFI1B. Interacts with GFI1; prevents its ubiquitination and proteasomal degradation.

ARIH2,E3连接酶蛋白ARIH2抗体Subcellular Location:
Nucleus. Cytoplasm.

Tissue Specificity:
Widely expressed with higher expression in granulocytes.

Post-translational modifications:
Ubiquitinated. Ubiquitination promotes proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.

Database links:
UniProtKB/Swiss-Prot: O95376.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.