上皮细胞转化序列2(ECT2)重组蛋白

YB451Hu021
Epithelial Cell Transforming Sequence 2 (ECT2)
Organism: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
4th Edition (Revised in August, 2012)
上皮细胞转化序列2(ECT2)重组蛋白[ DESCRIPTION ]
Protein Names: Epithelial Cell Transforming Sequence 2
Synonyms: ECT2
Species: Human
Size: 100µg
Source: Escherichia coli-derived
Subcellular Location: Nucleus. Cytoplasm. cytoskeleton, spindle. Cleavage furrow.
Midbody. Cell junction, tight junction.
上皮细胞转化序列2(ECT2)重组蛋白[ PROPERTIES ]
Residues: Glu453~Ile647 (Accession # Q9H8V3), with N-terminal His-Tag.
Grade & Purity: >95%, 24 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
Endotoxin Level: <1.0 EU per 1µg (determined by the LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass: 23.8 kDa
Predicted isoelectric point: 6.4
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
上皮细胞转化序列2(ECT2)重组蛋白degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-EGQRGGPI LAPEEIKTIF GSIPDIFDVH TKIKDDLEDL IVNWDESKSI GDIFLKYSKD LVKTYPPFVN
FFEMSKETII KCEKQKPRFH AFLKINQAKP ECGRQSLVEL LIRPVQRLPS VALLLNDLKK HTADENPDKS TLEKAIGSLK
EVMTHINEDK RKTEAQKQIF DVVYEVDGCP ANLLSSHRSL VQRVETI