肿瘤坏死因子受体超家族成员4(TNFRSF4)重组蛋白

YB519Mu022
Recombinant Tumor Necrosis Factor Receptor Superfamily,
Member 4 (TNFRSF4)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 肿瘤坏死因子受体超家族成员4(TNFRSF4)重组蛋白[ PROPERTIES ]
Residues: Lys29~Gly210 (Accession # P47741),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.5
Predicted Molecular Mass: 21.7kDa
 肿瘤坏死因子受体超家族成员4(TNFRSF4)重组蛋白Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 肿瘤坏死因子受体超家族成员4(TNFRSF4)重组蛋白[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-KH TYPSGHKCCR ECQPGHGMVS RCDHTRDTLC HPCETGFYNE
AV NY D TCKQC TQ CNHRSGSE LKQN CTPTQD TV C RCRPG TQ PR QDSGYK LG
VDCVPCPPGH FSPGNNQACK PWTNCTLSGK QTRHPASDSL DAVCEDRSLL ATLLWETQRP
TFRPTTVQST TVWPRTSELP SPPTLVTPEG
[ REFERENCES ]
1. Calderhead D.M., et al. (1993) J. Immunol. 151:5261-5271. 2. Birkeland M.L., et al. (1995) Eur. J. Immunol. 25:926-930. 3. Pippig S.D., et al. (1999) J. Immunol. 163:6520-6529. 4. Kopf M., et al. (1999) Immunity 11:699-708.