ETL,EGF毒素受体7跨膜结构域蛋白1抗体

产品编号HZ-13111R
英文名称ETL
中文名称EGF毒素受体7跨膜结构域蛋白1抗体
别 名EGF; EGF latrophilin and seven transmembrane domain containing 1; EGF TM7 latrophilin related protein; EGF-TM7-latrophilin-related protein; EGFTM7 latrophilin related protein; ELTD 1; ELTD1; ELTD1_HUMAN; ETL; ETL protein; KPG_003; latrophilin and seven transmembrane domain-containing protein 1; zgc:63629.
说 明 书0.1ml 0.2ml
研究领域细胞生物 信号转导 G蛋白偶联受体 G蛋白信号 跨膜蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Horse,
ETL,EGF毒素受体7跨膜结构域蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ETL/ELTD1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ETL,EGF毒素受体7跨膜结构域蛋白1抗体PubMedPubMed
产品介绍background:
The epidermal growth factor (EGF)-TM7 family constitutes a group of leukocyte-restricted, class B G-protein coupled receptors (GPCRs). These include CD97, EMR1(EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL. These family members are characterized by an extended extracellular region with several N-terminal EGF domains and are predominantly expressed on cells of the immune system. Unlike other GPCRs, neither EMR2 nor EMR3 have mouse orthologs. The molecular twins CD97 and EMR2 only differ by 6 out of 236 amino acids, but this slight difference is enough to alter ligand specificity and confer nonredundant functions. EMR3 may function in myeloid-myeloid interactions during immune and inflammatory responses. ETL is a 738 amino acid protein composed of a large extracellular domain with EGF-like repeats, a seven-transmembrane domain, and a short cytoplasmic tail. ETL mRNA expression is up-regulated in the adult rat and human heart.

Function:
Could be involved in cardiac development.

Subunit:
Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Forms stable dimer at the cells surface (By similarity).

ETL,EGF毒素受体7跨膜结构域蛋白1抗体Subcellular Location:
Cell membrane.

Tissue Specificity:
Mainly expressed in smooth muscle.

Post-translational modifications:
Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.

Similarity:
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 2 EGF-like domains.
Contains 1 GPS domain.

Database links:
Entrez Gene: 64123 Human
SwissProt: Q9HBW9 Human
Unigene: 132314 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat uterus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(HZ-13111R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: rat uterus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(HZ-13111R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: rat uterus tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(HZ-13111R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining