P53抗体Applications
| In-house applications |
ELISA, IP, WB, IF, IHC Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, WB |
Publications |
59 citations –view here |
| Species specificity |
Human, Rat, Mouse; other species not tested. |
| Published Species |
Human, mouse, rat |
|
Images
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 WB result of P53 antibody (10442-1-AP, 1:2000) with si-p53 and si-Control transfected A431 cells. MCF7 cells were subjected to SDS PAGE followed by western blot with 10442-1-AP(P53 antibody) at dilution of 1:1000 IP Result of anti-P53 (IP:10442-1-AP, 4ug; Detection:10442-1-AP 1:1000) with A431 cells lysate 3000ug. Immunohistochemical of paraffin-embedded human endometrial cancer using 10442-1-AP(P53 antibody) at dilution of 1:1000 (under 40x lens) Immunofluorescent analysis of HepG2 cells using 10442-1-AP( P53 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|
| Positive WB detected in |
MCF-7 cells, A431 cells, MCF7 cells, mouse colon tissue, rat colon tissue |
| Positive IP detected in |
A431 cells |
| Positive IHC detected in |
Human endometrial cancer tissue, human kidney tissue, human lung cancer tissue, human lung tissue, human ovary tumor tissue, human placenta tissue, human skin tissue, human testis tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:500-1:2000
IF : 1:20-1:200 |
P53抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
P53 fusion protein ag0698 |
Full name |
Tumor protein p53 |
Calculated molecular
weight |
44 kDa |
Observed molecular
weight |
53 kDa |
GenBank accession
number |
BC003596 |
Gene ID (NCBI) |
7157 |
| Gene symbol |
TP53 |
Synonyms |
Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
Background
P53抗体
|
TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein. P53抗体
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