Description

The most abundant cell type in the lung interstitium is the fibroblast. These fibroblasts resemble ordinary fibroblasts, but also possess some distinguishing features, such as long, branching processes and gap junctions. Pulmonary fibroblasts function to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. They play an important role in the repair and remodeling processes following tissue damage. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury [1]. An inadequate or an excessive accumulation of fibroblasts can result in abnormal tissue function. For example, excess proliferation of fibroblasts contributes to adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension [2]. 

 

MPF from ScienCell Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse lung. MPF are cryopreserved at passage 1 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MPF are characterized by immunofluorescence with antibody specific to fibronectin. MPF are negative for mycoplasma, bacteria, yeast, and fungi. HPF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

 

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPF in vitro.

 

Product Use

MPF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

 

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

 

Shipping

Dry ice.

 

Reference

[1] Kuwano K, Hagimoto N, Hara N. (2001) “Molecular mechanisms of pulmonary fibrosis and current treatment.” Curr Mol Med. 1: 551-73.

[2] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) “Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia.” Am J Physiol Lung Cell Mol Physiol. 282: L976-86.